Abstract The discovery and characterization of antigen-specific CD8+ T cell clonotypes typically involves the labor-intensive synthesis and construction of peptide-MHC tetramers. We adapt single-chain trimer (SCT) technologies into a high throughput platform for pMHC library generation. showing that hundreds can be rapidly prepared across multiple Class I HLA alleles. We use this plat... https://sportsplazanyes.shop/product-category/collections/